|Hands-On Proteomics Workshop||Dates and Registration|
Participants will learn introductory proteomics science and applicable protocols and technologies through a comprehensive, hands-on exploration of the various proteomics workflows. This course is appropriate for individuals with little to no experience in mass spectrometry and/or high performance liquid chromatography (HPLC) who desire a comprehensive overview of the typical proteomics workflows, including gel-based and on-line methods. Individuals who may primarily benefit from this course include: investigators who are considering incorporating proteomics protocols into their own research, graduate students and post-doctoral fellows, proteomics core users, industry sales representatives, bioinformaticists, and others who need to be familiar with typical proteomics workflows.
Lecture Topics: Introduction to Mass Spectrometry, Introduction to Database Searching, Front-End Technologies, Advanced Techniques, Spectral Interpretation, Clinical Proteomics, and Quantitative Proteomics. A Roundtable Discussion Session is also included.
Laboratories are divided into 3 stations where participants receive hands on experience in:
Sample Preparation: 1D gels, spot picking, in-gel digestion and cleanup, 2D gels (demonstration and mini-lecture), phosphopeptide enrichment, off-line fractionation techniques, and immunodepletion,
Instrumentation: HPLC, Nano-LC/MS, Quadrupole-TOF, ESI-TOF, HPLC-Chip MS, and QQQ. Curriculum includes: hardware and software overview, calibration (TOFs), gradient design (LC methods), and hands-on experience on each instrument. Students analyze their own peptide digests using multiple instruments, which were accumulated during the Sample Preparation laboratories.
Proteomics Informatics:A hands on spectral interpretation lab is included. Proteomic data informatics includes tools for large data set analysis such as Scaffold, Ingenuity, and Quantitative software such as GenespringMS.
Database Searching:The course begins with both lecture and hands-on experience with database searching. The course culminates with a ½ day database searching laboratory where students analyze data they themselves accumulated during the course. Includes: Basic MS and MS/MS searching using MASCOT and Spectrum Mill, and spectral review and validation.
Administrative information: We have arranged for reduced rates at several excellent area hotels; however, accommodations are the responsibility of the participant. Continental breakfast, snacks, and lunches are provided. Participants will receive a comprehensive course manual and disk containing all lectures and laboratories, data, and additional protocols.
Dates and Registration
1400 Jackson St., K924
National Jewish Health
Denver, CO 80206