Hands-On Clinical Proteomics

Clinical Proteomics Dates and registration
Students will learn introductory proteomics protocols and technologies in the context of specific clinical interests through a targeted, hands-on exploration of the various proteomics workflows. This course is designed for clinicians and others who are interested in using proteomics and/or mass spectrometry in their studies but are not sure how to get started. Non-clinicians who would also simply like to know more about the field of proteomics are also encouraged to attend. The course length is significantly shortened to meet the needs of the busy clinician or executive. Specific target audience: MD's (residents and faculty), PhD's, clinical study directors, executives.

Lecture Topics: Introduction to Mass Spectrometry, Introduction to Database Searching, Front-End Technologies, Web Resources, Clinical Proteomics (Sample collection/handling, sample preparation, clinical workflows, data output and management, biomarker discovery), Clinical Applications (Review of current/recent studies).

Laboratories are divided into 3 stations where participants receive hands on experience in:

Sample Preparation: spot picking, in-gel digestion and sample clean up, 1D and 2D gels (demonstration and mini-lecture).

Instrumentation I: HPLC-Chip MS/MS (ion trap): hardware and software overview, gradient design, analysis of standards, review of columns and solvents and demo of nanoLCMS. ESI-TOF

Practical Proteomics: Off-line fractionation technique: Immunodepletion (limited hands on includes entering parameters, project assignment and review), protein quantitation techniques, Spectral interpretation (with homework assignment and review).

Database Searching: The course culminates with a day database searching laboratory where students analyze data they themselves accumulated during the course. Includes: Basic MS and MS/MS searching using MASCOT and Spectrum Mill, and Spectral review and validation.

Dates and registration
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1400 Jackson St., K924
National Jewish Health
Denver, CO 80206